pcmv4 apoe3 (Addgene inc)
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pcmv4 apoe3
Pcmv4 Apoe3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv4+apoe3/pCMV4-ApoE3+(Plasmid+%2387086)/pmc12780660-62-57-61
Average 94 stars, based on 11 article reviews
Pcmv4 Apoe3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv4+apoe3/pCMV4-ApoE3+(Plasmid+%2387086)/pmc12780660-62-57-61
Average 94 stars, based on 11 article reviews
pcmv4 apoe3 - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Müller Glial Kir4.1 Channel Dysfunction in APOE4 ‐ KI Model of Alzheimer's Disease"
Article Title: Müller Glial Kir4.1 Channel Dysfunction in APOE4 ‐ KI Model of Alzheimer's Disease
Journal: Glia
doi: 10.1002/glia.70119
Figure Legend Snippet: APOE4 causes deficits in the Kir4.1. (a) Representative images of retinal slices showing Glutamine synthase (GS) and Kir4.1 staining pattern in APOE3 and APOE4 mice, scale 20 μm ( n : APOE3 = 3, APOE4 = 3). (b) Bar graph showing quantification of immunofluorescence for Kir4.1 and GS ( n : 11–12 images/group). (c) Representative current traces of Kir4.1 from freshly isolated Müller cells from APOE3 and APOE4 mice with and without 1 mM BaCl 2 treatment. Currents were elicited by a 50‐ms hyperpolarization to −140 mV from a holding potential of −60 mV. The dashed line indicates the closed state (zero current), the downward pulses represent channel openings, corresponding to inward K + current. The flickers indicate channel opening and closing. (d) Representative current–voltage (I–V) relationship of whole‐cell voltage‐gated K + currents of Kir4.1 from freshly isolated Müller cells from APOE3 and APOE4 mice with and without 1 mM BaCl 2 treatment. (e) Current densities of Kir4.1 from freshly isolated Müller cells from APOE3 and APOE4 mice collected from +30 mV ( n : APOE3 = 26 cells/9 mice, APOE4 = 33 cells/8 mice). Values are expressed as mean ± SEM. An unpaired t ‐test was used for statistical analysis. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Techniques Used: Staining, Immunofluorescence, Isolation
Figure Legend Snippet: Mitochondrial dysfunction in APOE4 . (a) Representative images of retinal slices showing glutamine synthase (GS) and TOMM20 staining pattern in APOE3 and APOE4 mice, scale 20 μm ( n : APOE3 = 3, APOE4 = 3). (b) Bar graph showing quantification of immunofluorescence for TOMM20 and GS ( n : 10–11 images/group). Values are expressed as mean ± SEM. An unpaired t ‐test was used for statistical analysis. * p < 0.05, *** p < 0.001.
Techniques Used: Staining, Immunofluorescence
Figure Legend Snippet: APOE4 decreases Kir4.1 and mitochondrial expression in rMC‐1. (a) Schematic showing the generation of rMC‐1 expressing human APOE isoforms. rMC‐1 was transiently transfected with human APOE2 / APOE3 / APOE4 , and EV was used as a control. (b) mRNA expression of Kcnj10 gene for Kir4.1 normalized to a housekeeping gene β‐actin. (c) Representative western blots of Kir4.1 expression and (d) quantification of integrated optical density (IOD) ratio of Kir4.1 and α‐tubulin showing decreased protein expression of Kir4.1 in APOE4 ‐transfected rMC‐1. (e) Representative images of rMC‐1 transfected with human APOE2 / APOE3 / APOE4 /EV showing decreased TOMM20 staining pattern in APOE4 ‐transfected rMC‐1, scale: 20 μm ( n : 3 independent experiments). (f) Quantification of TOMM20 staining intensity per cell area ( n : 15–24 cells/condition). (g) mRNA expression of Mfn1 , Mfn2 , and Dnm1 , showing that APOE4 ‐transfected rMC‐1 reduced Mfn1 , Mfn2 , and Dnm1 gene expression as compared to EV/ APOE2 / APOE3 ‐transfected rMC‐1 ( n : 4 independent experiments). Values are expressed as mean ± SEM. One‐way ANOVA followed by Tukey's multiple comparison test was used for statistical analysis. * p < 0.05, ** p < 0.01, **** p < 0.0001.
Techniques Used: Expressing, Transfection, Control, Western Blot, Staining, Gene Expression, Comparison
Figure Legend Snippet: APOE4 impairs mitochondrial respiration and reduces metabolic flexibility in rMC‐1. (a) OCR traces in rMC‐1 expressing EV/ APOE2 / APOE3 / APOE4 in response to sequential addition of oligomycin (oligo), FCCP, and rotenone/antimycin A (Rot/AA). APOE4 expressing rMC‐1 showed consistently lower OCR across conditions. (b) Quantification of basal respiration, maximal respiration, and non‐mitochondrial respiration, with APOE4 expressing rMC‐1 showing significantly reduced maximal and non‐mitochondrial respiration. (c) Quantification of spare respiratory capacity, ATP‐linked respiration, and proton leak. APOE4 ‐expressing rMC‐1 exhibited a marked reduction in spare respiratory capacity, while ATP‐linked respiration showed a downward trend. (d) ECAR profile in rMC‐1 expressing EV/ APOE2 / APOE3 / APOE4 in response to oligomycin (oligo), FCCP, and rotenone/antimycin A (Rot/AA) shows comparable basal rates across groups. (e) Quantification of glycolytic reserve, basal, and maximal ECAR. APOE4 rMC‐1 displayed a significantly reduced glycolytic reserve compared to EV, APOE2 , and APOE3 ‐transfected rMC‐1. (f) Quantification of glycolytic capacity and non‐glycolytic ECAR showing no significant changes across groups. (g) PPR traces in rMC‐1 expressing EV/ APOE2 / APOE3 / APOE4 in response to oligomycin (oligo), FCCP, and rotenone/antimycin A (Rot/AA) show overall comparable levels across groups. (h) Quantification of basal and maximal PPR confirms no significant APOE isoform differences. (i) Quantification of glycolytic PPR and non‐glycolytic PPR also showing no significant differences across groups ( n : 3 independent experiments, with 3–4 technical replicates per condition). Values are expressed as mean ± SEM. One‐way ANOVA with Tukey's test was used for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Expressing, Transfection
Figure Legend Snippet: MitoQ restores Kir4.1 gene and protein expression in rMC‐1 transfected with APOE4 . (a) mRNA expression of Kcnj10 gene for Kir4.1 normalized to housekeeping gene for β‐actin after treating rMC‐1 with 1 μM MitoQ and vehicle. mRNA expression of Kir4.1 was significantly increased in APOE4 ‐transfected rMC‐1 upon treatment with 1 μM MitoQ compared to the vehicle. (b) Representative western blots of Kir4.1 expression and quantification of IOD ratio of Kir4.1 and α‐tubulin showing comparable protein expression of Kir4.1 in APOE4 ‐transfected rMC‐1 as compared to EV/ APOE2 /APOE3‐transfected rMC‐1 after treating with 1 μM MitoQ. Values are expressed as mean ± SEM. Two‐way ANOVA followed by Tukey's multiple comparison test was used for statistical analysis. * p < 0.05, ** p < 0.01. ( n : 3–4 independent experiments).
Techniques Used: Expressing, Transfection, Western Blot, Comparison
Figure Legend Snippet: MitoQ decreases mitochondrial ROS in APOE4 ‐transfected rMC‐1. Representative images of unstained rMC‐1 and rMC‐1 transfected with EV/ APOE2 / APOE3 / APOE4 and treated with (a) vehicle or (b) MitoQ (1 μM). Cells were analyzed on a flow cytometer with 610/20 nm bandpass emission filter. (c) Bar graph showing quantification of % of MitoSox Red positive cells. Mitochondrial reactive oxygen species (ROS) was decreased upon treating APOE4 ‐transfected rMC‐1 with 1 μM MitoQ. Values are expressed as mean ± SEM ( n : 3 independent experiments). One‐way ANOVA followed by Tukey's multiple comparison test was used for statistical analysis. * p < 0.05, ** p < 0.01.
Techniques Used: Transfection, Flow Cytometry, Comparison
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